Journal: Cancers

Article Title: Vitamin A Rich Diet Diminishes Early Urothelial Carcinogenesis by Altering Retinoic Acid Signaling

doi: 10.3390/cancers12071712

Figure Lengend Snippet: Differential expression of genes involved in retinoic acid signaling in mouse bladder tissue following BBN treatment and vitamin A rich diet. Comparisons of NT ( n = 9), BBN ( n = 9), and BBN + VitA ( n = 9) groups of animals. ( A ) Heatmap representation of fold change in gene expression in BBN vs. NT and BBN + VitA vs. NT. ( B , C ) Volcano plots showing comparison of BBN vs. NT and BBN + VitA vs. NT. For each group, three cDNAs, each containing RNA from three different mice, were used. Significantly affected gene expression with fold change greater then 2, upregulated or downregulated, is marked in red or blue, respectively ( p value < 0.05; tested by two-sided Student’s t -test). FC, fold change.

Article Snippet: Data are expressed as Log2 fold change (FC) in heatmap and volcano plots using GraphPad Prism 8.

Techniques: Quantitative Proteomics, Gene Expression, Comparison

Journal: Cancers

Article Title: Vitamin A Rich Diet Diminishes Early Urothelial Carcinogenesis by Altering Retinoic Acid Signaling

doi: 10.3390/cancers12071712

Figure Lengend Snippet: Differential expression of genes involved in retinoic acid signaling in mouse bladder tissue after BBN treatment with or without vitamin A rich diet. Comparisons of NT ( n = 9), VitA ( n = 9), BBN + VitA ( n = 9), and BBN ( n = 9) groups. ( A ) Heatmap representation of fold change in gene expression in VitA vs. NT and BBN + VitA vs. BBN groups. ( B , C ) Volcano plots showing comparison of VitA vs. NT and BBN + VitA vs. BBN groups. For each group, three cDNAs, each containing RNA from three different mice, were used. Significantly affected gene expression with fold change greater then 2, upregulated or downregulated, is marked in red or blue, respectively ( p value < 0.05; tested by two-sided Student’s t test). FC, fold change.

Article Snippet: Data are expressed as Log2 fold change (FC) in heatmap and volcano plots using GraphPad Prism 8.

Techniques: Quantitative Proteomics, Gene Expression, Comparison

Journal: bioRxiv

Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes

doi: 10.1101/2025.04.09.647991

Figure Lengend Snippet: (A) ( A ) A diagram depicting the generation of SLC25A51-floxed C57BL/6N mice using a CRISPR-Cas9-based approach by Biocytogen Pharmaceuticals. ( B, C ) SLC25A51 expression per cell was compared between T2D patients and healthy controls using two different human beta-cell scRNA-seq datasets (E-MTAB-5061, GSE81608) ( Table S3 ). Heatmaps of Cdkn1a (p21) and Cdkn2a (p16) expression were also analyzed in both datasets. ( D ) 30-week-old SLC25A51-floxed C57BL/6N male mice were infused with either AAV6-INS1-Empty or AAV6-INS1-CRE viruses via pancreatic ductal infusion. Mice were sacrificed 15 weeks post-surgery. SLC25A51, p16 INK4A , MTCO2, and beta-actin protein expression levels were analyzed in pancreatic beta cells isolated from 45-week-old INS1-EMPTY (n=2) and INS1-CRE (n=4) groups. ( E-K ) Pancreatic ductal infusion of either PBS, AAV6-INS1-Empty, or AAV6-INS1-CRE was performed in each group of female C57BL/6N mice (n=5–6 per group). ( F ) Random blood glucose levels and ( G ) body weight were measured twice a week, and ( H ) beta-hydroxybutyrate levels were measured once a week for each mouse. ( I-K ) Six or eight weeks post-surgery, female mice underwent an IPGTT and ITT. Graphs show blood glucose levels at ( I, J ) each time point and area under the curve. ( I ) During the IPGTT, serum was collected from each group at 0 and 15 min to measure ( K ) serum insulin levels. Two weeks after the IPGTT, ( J ) an ITT was performed to test insulin sensitivity. Two-way ANOVA; error bars represent SEM; **p < 0.01.

Article Snippet: The data were visualized using heatmaps or dot-plot graphs in GraphPad Prism.

Techniques: CRISPR, Expressing, Isolation